Studies on Serum Antitrypsin

The Nature of Serum Antitrypsin

1. The ferment-inhibiting action of the serum is due to the presence of compounds of the unsaturated fatty acids. 2. These fatty acid compounds may be removed from the serum by means of chloroform or ether. 3. Soaps prepared by saponifying the chloroform or ether extracts inhibit the action of trypsin. 4. The anti-enzyme action of the serum can be removed by filtering acid serum through kaolin,...

Serum alpha 1 antitrypsin and pulmonary emphysema.

Using isoelectric focusing (IEF) and radial immunodiffusion (RID) techniques, serum samples from 100 normal healthy adults and 21 patients with pulmonary emphysema were analysed to identify various alpha 1 antitrypsin phenotypes and the serum concentrations. Ten percent of the patients had low serum values. The normal or most common genetic form, MM, is the predominant phenotype in both control...

Estimation of the antitrypsin activity of serum.

A method for the estimation of serum trypsin inhibitory capacity (TIC) was developed to permit consistent results with various trypsin sources without further standardization. Very small volumes of serum are used and the results are expressed in international enzyme units. Normal women show a slightly higher mean value for TIC than normal men. Sixty-seven patients with pulmonary emphysema were ...

Genetic variants of serum alpha 1 antitrypsin.

Complete absence of data on alpha 1 antitrypsin in this country prompted us to determine serum levels using radial immunodiffusion (RID) and phenotypes by isoelectric focusing (IEF) in 100 healthy adults (52 males and 48 females). Mean serum alpha 1 antitrypsin concentration in healthy subjects was 2.47 +/- 0.08 g/l and the main phenotypes MM (70%), M1 M2 (28%) and FM 3 (2%) are infrequent in o...

Serum alpha 1 antitrypsin in liver diseases.